| 摘要 |
PURPOSE:To efficiently obtain a physiologically active peptide, by linking a physiologically active peptide having no lysine residues to a specific polypeptide through lysine residues to form a fused protein and cultivating Escherichia coli, etc., transformed with the fused protein. CONSTITUTION:A peptide having no lysine residues, e.g. alphahANP, is effectively produced by using a recombinant DNA technique. In the process, the peptide is linked to the C terminal of a polypeptide, containing a polypeptide fragment, derived from Escherichia coli-beta-galactosidase, having no lysine residues and consisting of 90-220 amino acids through lysine residues to give a fused protein. Escherichia coli transformed with the fused protein is then cultivated and the cultivated bacterial cells are crushed. The fused protein is solubilized from an insoluble fraction thereof with a solution of a solubilizing agent and extracted. The obtained fused protein is treated with lysyl endopeptidase to afford the desired peptide thereof. |
