Process for the preparation of LMW Heparin.

摘要

A method for production of low molecular weight heparin (LMW-heparin) by enzymatic batch depolymerization of heparin wtth heparinase wherein heparin is depolymerized to a desired weight average molecular weight by depolymerization to the corresponding number average molecular weight. The method enables depolymerization to a desired weight average molecular weight by measuring the increase in UV-absorption (preferably at 230-235 nm) DELTA A235 and halting the depolymerization when DELTA A235 has reached the calculated value corresponding to the desired number average molecular weight Mn.