MOUSE-HUMAN HETEROHYRIDOMAS WHICH SECRETE MONOCLONAL ANTIBODIES TO HUMAN ERYTHROCYTE RHESUS D ANTIGEN

摘要

The present invention provides an anti-Rhesus D (RhD) monoclonal antibody producing heterohybridoma formed by fusion of non-Ig secreting mouse myeloma cells with an anti-RhD Ig producing population of Epstein Barr virus (EBV)-transformed human B lymphocytes, which heterohybridoma when cultured at 37 DEG C in hybridoma culture medium (RPMI-l640 supplemented with l0% (v/v) fetal calf serum or a medium which sustains equal growth under the same conditions) at about l x l0<6> cells/ml produces more than 20 mu g anti-RhD monoclonal antibody/ml/24hrs. Two heterohybridomas which produce IgM anti-RhD monoclonal antibodies, designated MAD-2 and FOM-l, have been deposited at the European Collection of Animal Cell Cultures, UK under accession nos. 86041803 and 87021301.