IMMUNOLOGICAL MEASUREMENT OF ERYTHROPOIETIN

摘要

PURPOSE:To measure the density of erythropoietin (EPO) at a low cost in a short time with a high sensitivity in an oridinary inspection chamber, by using a labelled antibody comprising a specified antibody and a label substance. CONSTITUTION:An insoluble support to which is coupled a first antibody tended to react with EPO specifically is caused to react with a second antibody that is tended to react specifically with a liquid to be inspected containing EPO and EPO itself while obtained by immunization on an animal different from the first antibody and then, with a labelled antibody to measure the amount of the labelled antibody coupled onto the insoluble support or the amount of the labelled antibody not coupled thereonto. The labelled antibody is formed by bonding a label substance such as enzyme to a third antibody which will specifically react with immuno globulin of the animal from which the second antibody was prepared. With the bonding of the label substance onto the support proportional to the amount of EPO coupled, the EPO concentration of the liquid being detected is determined by comparison with a calibration curve prepared beforehand.