| 摘要 |
The present invention relates to a method for volumetric determination, dilution and transfer of liquid specimens, especially blood samples for counting corpuscles. The method comprises the steps of sucking up a relatively large amount of specimen, and of pressing, by means of a fixed volume of a reagent, a fixed volume of the sucked-up specimen into a mixing beaker, so as to obtain a mixture in a first dilution. According to the invention, the method comprises the steps of transferring, via a volume buffer, the mixture to a counting beaker by means of excess pressure in the mixing beaker. Before the entire mixture has been transferred from the mixing beaker, but after the mixture has entirely filled the volume buffer, the transfer from the mixing beaker is terminated. Any excess in the counting beaker is sucked off, and then the mixture in the volume buffer is flushed out into the counting beaker by means of a fixed volume of a hemolysing agent exceeding the volume of the buffer, so as to obtain a mixture in a second delution. After the flushing of the mixture has been terminated, the volume buffer is entirely filled with the hemolysing agent. The invention also relates to an apparatus for carrying out the method. |
