发明名称 |
Process for quantitative determination of substrate treated with oxidase |
摘要 |
A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent.
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申请公布号 |
US4695539(A) |
申请公布日期 |
1987.09.22 |
申请号 |
US19830516241 |
申请日期 |
1983.07.22 |
申请人 |
WAKO PURE CHEMICAL INDUSTRIES, LTD. |
发明人 |
SAKATA, YOSHITSUGU;MIYASHITA, YOSHIBONU;HAMANAKA, TADASHI;KODERA, HIROYUKI;MIKI, YUTAKA;YAMANISHI, KAZUHIKO;HANADA, TOSHIRO |
分类号 |
G01N33/50;C12Q1/26;C12Q1/28;C12Q1/54;C12Q1/60;C12Q1/62;(IPC1-7):C12Q1/62 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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