Prokaryotic expression system.

摘要

Hybrid proteins are prepared using a novel biological system comprising a prokaryotic host transformed with novel by hybrid plasmids beta -glucuronidase (BG) gene DNA and the desired protein gene DNA. Specifically exemplified are plasmids which comprise BG gene DNA and protein A DNA. E. coli K-12 derivative hosts transformed with plasmid pBG3-2 DELTA N express >60% of the desired fusion protein having protein A-like biological activity.