Competitive elisa for the detection of antibodies.

摘要

A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies is disclosed. This ELISA technique is more sensitive, more specific, and more accurate than known ELISA techniques. The competitive ELISA of this invention is particularly suited to the detection of human T-cell leukemia-lymphoma virus type III (HTLV-III). An improvement of the ELISA technique represented by the present invention lies in the use of a reaction buffer which suppresses non-specific adsorptive reactions between the test substrate and the test antigen. Furthermore, the ELISA technique is improved by removing contaminants from the target atnigen preparation.