摘要 |
PURPOSE:To make quantitative determination of a reducing material with high sensitivity by acting an reduction type Co complex to the reducing material to convert the same to an oxidation type Co complex then acting the same to a metallic indicator and measuring the amt. of the formed chromatic complex. CONSTITUTION:The reducing material as a material to be inspected, the metallic indicator and oxidation type Co complex are added into a buffer soln. having prescribed pH (5-10) at a specified temp. to act the Co complex to the reducing material and to convert the same to the reduction type Co complex, then said complex is acted with the metallic indicator to form the chromatic complex. The reducing material is quantitatively determined by measuring the amt. of the chromatic complex by an optical means. The oxidation type Co complex is so added that the concn. in the reaction liquid attains 0.01-10mmol/l, more preferably 0.05-1mmol/l. The metallic indicator is used in a concn. range of 0.01-200mmol/l, more preferably 50-500mumol/l.
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