摘要 |
<p>The present invention provides a novel apparatus for culturing mammalian cells, whereby a plastic housing (1) encasing a double membrane bag or bags (11) supplies nutrients and oxygen to mammalian cells for propagation thereof. The double membrane bag contains a first bag (25,26) formed of a microfiltration or ultrafiltration membrane disposed within a second membrane bag (24,27) formed of a liquid impermeable, gas permeable membrane. This apparatus has a first chamber (43) containing oxygen, a second chamber (50) containing cells and a third chamber (28) containing nutrients. </p> |