发明名称 Process for regenerating soybeans
摘要 The present invention relates to the regeneration of soybeans. The process comprises the steps of: (a) culturing tissue obtained from a soybean plant on a first medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure callus and embryoid formation; (b) subculturing the embryoids on a second medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure embryoid maturation; (c) subculturing the embryoids on a third medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure shoot formation, and (e) subculturing the shoots on a fourth medium comprising mineral salts, vitamins and sucrose in an amount sufficient to ensure root formation.
申请公布号 US4684612(A) 申请公布日期 1987.08.04
申请号 US19840635222 申请日期 1984.07.27
申请人 SUNGENE TECHNOLOGIES CORPORATION 发明人 HEMPHILL, JOHN K.;EIKENBERRY, ERIC J.
分类号 A01H4/00;(IPC1-7):C12N5/00;C12N5/02 主分类号 A01H4/00
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