| 摘要 |
A method to prepare pure pertussis vaccine is described. Thus, Bordetella Pertussis Tohama Phase I is inoculated into stainer- scholte medium. After incubation, the culture broth is satd. with 33% (NH4)2SO4 and stood for 6 days at 4≦̸C. After centrifuged at 10000 rpm for 20 min, the resulting ppt.is dissolved in phosphate buffer, satd. with 50% (NH4)2SO4, and stood for 3 days at 4≦̸C. After centrifuged and dialized, the resulting soln. is centrifuged at 4≦̸C and 37000 rpm for 18h by sucrose density gradient. The obtained fraction is treated with formalin (0.3--0.4%) detoxified at 37≦̸C for 2 wks, and dialized to give pure pertussis vaccine.
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