| 摘要 |
In a biospecific multianalyte assay the use of microspheres and fluorescent labels with substantially different fluorescence decay times, is combined. The assay is performed in a suspension of microspheres in the form of a pool of different microsphere categories, where the categories represent different analytes. The microspheres belonging to the respective categories are first coated with a specific reactant, i.e. the microspheres function as a solid support for the reactant and for a biospecific reaction. Fluorescent labels having a short decay time are used to identify the category of each individual microsphere, while fluorescent labels having a long decay time are used to determine the concentration of a particular analyte on the microsphere by means of the biospecific reaction. |
