| 摘要 |
The present invention relates to the regeneration of sunflowers via embryogenesis and organogenesis. The process comprises the steps of: (a) culturing tissue obtained from a sunflower plant on a first medium which comprises mineral salts, vitamins, amino acids, sucrose and a hormone in an amount sufficient to ensure callus formation; (b) optionally subculturing said callus on a maintenance medium which comprises mineral salts, vitamins, amino acids, sucrose and a hormone in an amount sufficient for callus maintenance; (c) subculturing said callus on a second medium which comprises mineral salts, vitamins, amino acids, sucrose and a hormone in an amount sufficient to precondition the callus; (d) subculturing said callus on a third medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure shoot formation, and (e) subculturing said shoot on a fourth medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure root formation, whereby plants are obtained.
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