EXPRESSION OF SUPEROXIDE DISMUTASE IN EUKARYOTIC CELLS

摘要

The invention concerns the production of superoxide mutase (SOD) by eucaryotic cells derived from multicellular organisms which are capable of expressing a DNA sequence encoding for superoxide dismutase and producing the superoxide dismutase or analogs thereof. A specific embodiment of the invention includes a human HeLa cell or mouse L cell containing either the pmSV2-SOD-Neo plasmid or the pHG-SOD-SV-Neo plasmid. The pmSV2-SOD-Neo plasmid contains a DNA sequence encoding for superoxide dismutase joined to the SV40 early promoter. The pHG-SOD-SV-Neo plasmid contains a DNA sequence encoding for superoxide dismutase controlled by the native regulatory elements of human genomic Cu-Zn SOD. Such cells are capable of expressing the sequence encoding the superoxide dismutase and of producing the superoxide dismutase or an analog thereof. The invention also concerns methods of producing the superoxide dismutase by growing the cells of the invention and recovering the superoxide dismutase product.