摘要 |
<p>There is disclosed a container for the insemination of human ovocytes in the absence of CO2-enriched air which can be carried out in an incubator at 37<o>C or in the vaginal cavity. Said cylindrical container has smooth and round external walls (1) of which the thickness is equal to 2 mm at the cylinder body (2) and decreases down to 1 mm at the neck (3). On the external wall of the neck there is provided a thread (4) for screwing externally the plug (12) of which the edges are smooth and round. The blind extremity of the container is round (5) whereas the other is flat (6) and has a round opening (7) which is closed by a thin membrane (9) to provide for hermeticity of the tube in order to avoid pertubations of the culture medium (8) present in the container as early as its fabrication. Above the liquid there remains approximatly 200 ul of gas (10). The internal walls are smooth and rounded (11). After deposition of the ovocytes and spermatozoids required for the insemination and, therefore after rupture of the membrane, hermeticity is obtained after screwing the plug through two seals, one of them being circular to the neck of the tube (13) and the other one being flat and round (14) at the bottom of the plug and which will be flattened out on the opening of the tube (7) by a projection situated at the bottom of the plug (15) and of which the diameter is substantially equal to that of the opening. The container will be held in the cul de sac of the vaginal cavity by means of a flexible ring comprised of a metal strip (17) shielded with rubber (18) to which is secured a rubber pouch (19) wherein the container is placed. Said device provides for the intravaginal insemination of human ovocytes in the absence of CO2-enriched air, the vaginal cavity replacing the conventionally used incubator.</p> |