| 摘要 |
PURPOSE:To effectively compensate the variation of a light source by dispensing with an exclusive monitoring detector, by allowing a part of irradiation light passed through a flow cell to be incident to a measuring photomultiplier tube and performing the negative high voltage feedback of the photomultiplier tube on the basis of the signal corresponding to the irradiation light in an output signal. CONSTITUTION:The light from a light source 2 is allowed to irradiate the sheath flow cell 14 of a system through a wavelength selecting filter 4, a lens 6, a slit 8 and a lens 12 and the fluorescence from the particles flowing through said cell 14 is detected by a lens 24 to be incident to a fluorescence detecting photomultiplier tube 28 through a wavelength selecting filter 26. The scattered light due to the particles flowing through the cell 14 is condensed by a lens 16 to be applied to a scattered light detecting photomultiplier tube 22 through a wavelength selecting tube 20. A translucent beam stopper 50 is provided on the optical axis of the lens 16 and a part of scattered light is incident to a photomultiplier tube 28. The signal corresponding to the irradiation light from the photomultiplier tube 22 is processed by an irradiation light signal separation circuit 52 and a negative high voltage feedback circuit 32 and the output of a negative high voltage generating circuit 34 is applied to the photomultiplier tubes 22, 28. |
