摘要 |
<p>PURPOSE:To mass produce calluses colored in red, by cultivating cells of safflower by two-stage cultivation of solid culture and liquid culture and changing concentration of culture components. CONSTITUTION:Cells or a cell group of safflower is cultivated in a conventional solid medium. Then, the prepared calluses are cultivated in a liquid medium comprising components having reduced concentration of at least one component of the solid medium components, preferably one or more selected from an inorganic component, plant hormone, vitamin and amino acid.</p> |