发明名称 |
Method of somatotropin naturation |
摘要 |
Methods of solubilization and naturation of somatotropin protein from refractile bodies of host cells are disclosed. The solubilization methods embrace the discovery that an aqueous urea solution can be effectively used to solubilize refractile bodies containing such somatotropin protein. Surprisingly, it has been further found that once solubilized, such somatotropin protein can be natured in the urea solution by contacting the solution with a mild oxidizing agent for a time sufficient to result in the formation of the disulfide protein. Naturation can efficiently occur even at high protein concentration, in an impure preparation and in the absence of reducing agent.
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申请公布号 |
US4652630(A) |
申请公布日期 |
1987.03.24 |
申请号 |
US19850704677 |
申请日期 |
1985.02.22 |
申请人 |
MONSANTO COMPANY |
发明人 |
BENTLE, LARRY A.;MITCHELL, JAMES W.;STORRS, STEPHEN B. |
分类号 |
C07K1/113;C07K14/61;(IPC1-7):C07K1/02 |
主分类号 |
C07K1/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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