发明名称 |
Process for the preparation of 5'-ribonucleotides |
摘要 |
Ribonucleic acid in a mixture of crude nucleic acids is selectively hydrolyzed with 5'-phosphodiesterase to 5'-ribonucleotides without hydrolyzing desoxyribonucleic acid in the mixture. Selective hydrolysis is carrying out by contacting the crude nucleic acid mixture with 5'-phosphodiesterase immobilized on a polymer carrier. The crude mixture of nucleic acids is preferably obtained by aqueous extraction of microorganisms that have previously been extracted with ammonia and a lower alcohol to remove lipids. The polymer carrier is preferably a copolymer of glycidylmethacrylate, allylglycidylether, methacrylamide and methylene-bis-methacrylamide.
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申请公布号 |
US4649111(A) |
申请公布日期 |
1987.03.10 |
申请号 |
US19850764967 |
申请日期 |
1985.08.12 |
申请人 |
HOECHST AKTIENGESELLSCHAFT |
发明人 |
KELLER, REINHOLD;SCHLINGMANN, MERTEN |
分类号 |
C12P19/32;C07H19/16;C07H19/20;C07H21/00;C12N11/08;C12N11/12;C12P19/30;(IPC1-7):C12P19/30;C12N11/02 |
主分类号 |
C12P19/32 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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