| 摘要 |
<p>PURPOSE:To obtain arbutin in high yield, by subjecting a callus of plant or a tumor tissue to subculture in a medium containing hydro-quinone. CONSTITUTION:A callus of plant of a tumor tissue is subjected to subculture in a medium containing <=1mM hydroquinone to induce a culture mixture having improved arbutin formation ability. A cell group of tissue piece of root, hypocoty or cotyledon of budding (young plant) of a plant such as Catharanthus roseus L. or root, stem, leaf, petiole, flower, pollen, etc. is of grown plant is used as a starting raw material and it is cultivated in a medium containing auxin or cytokinin by a conventional procedure to induce a callus. In this case, the callus is induced even by using the cell group or tissue of organ of any plant as a material although there is some difference in hardness. A medium obtained by blending Murashige & Skoog medium with agar is used s the medium but the medium is not limited thereto.</p> |
