| 摘要 |
PURPOSE:To achieve accurate temperature correction of a reaction current, by providing two reference electrodes on the exposed surface of a solid electrode to be used in the polarograph method or the like in addition to the working and opposed poles to measure the temperature with the two reference electrodes. CONSTITUTION:A working pole 53 and an opposed pole 54 are provided on the exposed surface of the body 60 of a solid electrode and a voltage is applied to both the poles to detect the reaction current. Two reference electrodes 51 and 52 different in the temperature coefficient of the electrode potential are provided. The difference DELTAE between potentials EA and EB of the electrodes 51 and 52 is expressed by DELTAE=EA-EB=a0+a1(t-25)+a2(t-25), wherein a1 and a2 are coefficients and (t) temperature. Here, with the coefficients a1 and a2 known, the temperature can be determined by measuring the difference DELTAE. Thus, the direct measurement of the temperature with two reference electrodes on the electrode surface enables the temperature measurement at the closest to an immobilized enzyme membrane as enzyme reaction field thereby assuring highly accurate measurement of the reaction current with a temperature correction. |
