| 摘要 |
NEW MATERIAL:A plasmid pSAN484, having 2.80+ or -0.05 megadaltons (hereinafter abbreviated to MD) molecular weight and one cleaved position (hereinafter abbreviated to site) by restriction enzymes BamHI, SacI, SacII, PstI and MluI and two sites by BclI and KpnI, the sites being as follows based on the site cleaved by BamHI as the coordinate point; SacI site at 0.91MD, SacII site at 2.09MD, PstI site at 2.19MD MluI site at 2.59MD, BclI sites at 0.69MD and 1.31MD and KpnI sites at 0.16 MD and 2.15MD and shown in the figure. USE:A recombinant DAN and cloned vector of exogenotes. PREPARATION:Streptomyces sp. SANK 60895 strain is cultivated to give mycelia, which are then subjected to bacteriolysis. The resultant bacteriolysis product is then treated by equilibrium density gradient centrifugation, etc., to afford a mixture of a plasmid pSAN484 with a plasmid pSAN483, which is then treated with a restriction enzyme, subjected to equilibrium density gradient centrifugation, dialyzed, etc., to isolate the aimed plasmid pSAN484. |
