| 摘要 |
PURPOSE:To make it possible to produce D-lactic acid of high purity even when batch fermentation is repeated, by supplying air to a culture medium in producing D-lactic acid using a microorganism, belonging to the genus Sporolactobacillus and capable of producing the D-lactic acid. CONSTITUTION:A microorganism, belonging to the genus Sporolactobacillus and capable of producing D-lactic acid, e.g. Sporolactobacillus inulinus ATCC15538, is cultivated to prepare a seed microorganism, which is then cultivated in a culture medium consisting of a saccharide, e.g. glucose or fructose, inorganic salt, growth promoting component, neutralizing agent, etc. The resultant fermentation liquor is partially used as a seed microorganism for the next fermentation to repeat the similar lactic acid fermentation. In the process, air is supplied into the fermentation culture medium, and the deterioration in purity of the D-lactic acid can be prevented. The volume of the air to be supplied is <10vol.% per min (0.1 V.V.M.) based on the amount of the culture fluid. |
