发明名称 |
PRODUCTION METHOD AND CRYOPRESERVATION METHOD FOR AMNIOTIC MESENCHYMAL CELL COMPOSITION, AND THERAPEUTIC AGENT |
摘要 |
An object of the present invention is to provide a method for producing a mesenchymal stromal cell composition, comprising conveniently and aseptically separating high-purity amnion-derived MSCs by performing enzyme treatment only once. According to the present invention, the following are provided: a method for producing a mesenchymal stromal cell composition, comprising: performing enzyme treatment of an amnion with collagenase and thermolysin and/or dispase; and filtering the enzyme-treated amnion through a mesh; a method for producing a cryopreserved mesenchymal stromal cell composition; and a therapeutic agent comprising as an active ingredient the mesenchymal stromal cell composition for a disease selected from graft-versus-host disease, inflammatory bowel disease, systemic lupus erythematosus, liver cirrhosis, or radiation enteritis. |
申请公布号 |
EP3037523(A1) |
申请公布日期 |
2016.06.29 |
申请号 |
EP20140837352 |
申请日期 |
2014.08.18 |
申请人 |
NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER;FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION;HYOGO COLLEGE OF MEDICINE;NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY;KANEKA CORPORATION |
发明人 |
YAMAHARA KENICHI;TAGUCHI AKIHIKO;SOMA TOSHIHIRO;OHNISHI SHUNSUKE;KOBAYASHI AKIRA |
分类号 |
C12N5/077;A61K35/50;A61P1/00;A61P1/16;A61P37/06;C12N1/04 |
主分类号 |
C12N5/077 |
代理机构 |
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