| 主权项 |
1. A method for producing cyclic adenosine monophosphate by whole-cell biocatalysis, the method comprising:
preparing a reaction solution comprising an enzyme activity protector, a substrate, an energy provider, a metal ion, and a permeable microbial strain and an organic solvent; and conducting a whole-cell biocatalysis reaction in said reaction solution to obtain cyclic adenosine monophosphate, wherein said enzyme activity protector is one or more polyhydric organic reagents selected from the group consisting of mannitol, maltitol, xylitol, lactitol, sorbitol, and glycol; said substrate comprises a cyclic adenosine monophosphate precursor and phosphate ion; and said energy provider is glucose; said metal ions are any one or more selected from magnesium ion and ammonium ion; said permeable microbial strain is any one strain selected from arthrobacter, microbaterium and corynebacterium; and said organic solvent is acetone; wherein the one or more polyhydric organic reagents is present in an initial reaction concentration of 0.001-10 g/L, the cyclic adenosine monophosphate precursor is present in an initial reaction concentration of 0.01-100 g/L, the phosphate on is present in an initial reaction concentration of 0.01-100 g/L, the glucose is present in an initial reaction concentration of 0.01-100 g/L, the metal ion is present in an initial reaction concentration of 0.001-10 g/L, the microbial strain is present in an amount of 10-1,000 g wet thallus/L, and the acetone is present in an initial reaction concentration of 0.01-50 mL/L. |
