| 摘要 |
PURPOSE:To produce novel serratia protease SSP-1, by using a recombinant vector integrating a DNA fragment producing serine protease present in a chromosome DNA of Serratia marcescens. CONSTITUTION:Novel serratia protease obtained by separating a DNA fragment producing serratia protease SSP-1 from the chromosome of Serratia marcescens IFO3046, preparing a recombinant vector from the DNA fragment and, e.g. pBR322, inserting the resultant recombinant vector into Escherichia coli C600 (r<->,m<->), preparing a microorganism producing serratia protease SSP-1 from the resultant transformant, cultivating the resultant microorganism producing the serratia protease SSP-1 in an ordinary nutrient culture medium and purifying the aimed serratia protease SSP-1 by applying separating and purifying means, e.g. salting out with ammonium sulfate, etc., chromatographic method with an adsorbent resin, etc., to give the aimed serratia protease SSP-1 of high purity. |
