Tissue-derived tumor growth inhibitors, methods of preparation and uses thereof

摘要

An acidified, ethanol extract derived from human tissue which comprises a plurality of acidic polypeptides, each of which has a molecular weight less than about 20,000 daltons and each of which has the property of inhibiting the growth of human tumor cells and of an established mink lung cell line - (CCL64) while stimulating the growth of normal human foreskin fibroblasts is disclosed. The inhibitory activity against human tumor cell growth is not destroyed upon increasing the temperature of the acidified, ethanol extract to about 100°C for about 3 minutes or adding acetic acid until the acidified, ethanol extract is up to about 1.0 molar in acetic acid. Further, the activity of the acidified, ethanol extract is enhanced when the acidified, ethanol extract is prepared at about 4°C rather than about at 23°C.

An acidified, ethanol extract derived from human umbilical cord which has been treated to removed substantially all blood, all extracellular soluble components and substantially all intracellular soluble components which comprises at least one acidic polypeptide having an apparent molecular weight less than about 30,000 daltons, compositions of matter designated tissue-derived growth inhibitor-1 (TGI-1), tissue-derived growth inhibitor (TGI), and tissue-derived growth inhibitor-2 (TGI-2) and a heterogeneous population of polypeptides designated CM-I, CM-II, CM-III and CM-IV, are also disclosed as are methods of preparation, pharmaceutical compositions which include, and uses of the foregoing.