| 摘要 |
PURPOSE:To obtain the result of measurement with good accuracy by absorbing once a sample to be measured in a dried porous material, disposing the same near a carrier for electrophoresis and introducing a sample thereto. CONSTITUTION:The sample is dropped through a sample injecting nozzle 2 onto a sample absorber 1 consisting of dry filter paper. A trisglycine buffer soln. is then poured through an injecting nozzle 3 and an injecting port 4 into an upper electrolytic cell 5 and a lower electrolytic cell 6 to prepare an upper electrolyte 7 and a lower electrolyte 8. A DC voltage is impressed between platinum electrodes (cathode) 10 and (anode) 11 from a DC power source. The various components in the sample absorber 1 are migrated to an antibody immo bilized film 12 fixed with the antibody for the component desired to be measured and only the measuring component is trapped in the film 12. The other com ponents pass through the film 12 and migrate into the lower electrolyte 8. An antibody soln. labeling luminol is then injected from a nozzle 13 onto the film 12 and the DC voltage is impressed thereto. The electrolytes 7, 8 are discharged and a soln. of a hypochlorous acid and H2O3 is injected onto the film 12. The quantity of the light emitted from the film 12 is measured by a photocounter.
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