Cloning and expression of delta-endotoxin genes of bacillus thuringiensis in bacterial cells and products therefrom.

摘要

The present invention arises from the discovery that the genes for the toxin crystal protein could be located on the endogenous plasmids of Bacillus thuringiensis, variety Kurstaki. Production of the toxin crystals in Bacillus subtilis is carried out using recombinant DNA technology, by channeling particular genes through Escherichia coli and to B. subtilis. Insect toxicity of the crystals has been demonstrated in laboratory tests.