| 摘要 |
<p>A process for producing a physiologically active substance by a combined enzymatic method is disclosed. In the combined enzymatic method, a reactant solution containing a precursor or precursors for the physiologically active substance, AXP, and a divalent metal ion is supplied at one end of a reactor incorporating either the combined enzymatic reaction system (a) or (b), wherein
<UnorderedLists id="ula01" listStyle="none"><ListItem>(a) is a reaction system including an enzyme for converting AMP to ADP, an enzyme for converting ADP to ATP, and an enzyme which catalyzes the synthesis of the physiologically active substance as it converts ATP to AMP; and</ListItem><ListItem>(b) is a reaction system including an enzyme for converting ADP to ATP and an enzyme which catalyzes the synthesis of the physiologically active substance as it converts ATP to ADP,</ListItem></UnorderedLists></p><p>Wherein the concentration of the divalent metal ion supplied into the reactor is held at a level no higher than 30mM while the concentration of the AXP is held below that of that of the precursor or precursors for the physiologically active substance, and the physiologically active substance produced is withdrawn from the other end of the reactor.</p><p>This process offers the following advantages:
<UnorderedLists id="ula02" listStyle="none"><ListItem>a physiologically active substance of interest can be synthesized in a continuous-flow bioreactor without forming any precipitate that will plug the reactor; and the maneuverability of the reactor and the yield of the physiologically substance of interest are appreciably increased.</ListItem></UnorderedLists></p> |
