RECOMBINANT DNA EXPRESSION VECTORS AND METHOD FOR GENE EXPRESSION

摘要

The present invention provides a process for preparing selectable and autonomously replicating recombinant DNA expression vectors which comprise 1) a transcriptional and translational activating sequence which is in the reading frame of a nucleotide sequence that codes for a peptide or polypeptide; 2) a translational stop signal; 3) a translational start signal which is in the reading frame of a nucleotide sequence that codes for a functional polypeptide; and 4) an additional translational stop signal. The above-described nucleotide sequences are engineered to generate, upon transcription, a translatable polycistronic mRNA. The invention further provides a method of use.