| 摘要 |
PURPOSE:To obtain a hyridoma capable of multiplying permanently a specific monoclonal antibody, by imparting ouabain resistance artificially to a fusible cell derived from human Burkitt's lymphomas. CONSTITUTION:A parent cell for imparting ouabain resistance is obtained by separating a cell capable of fusing with a cell producing an antibody in lymphoblasts separated from human Burkitt's lymphomas. For example, a cell designated as NAT-30 is used as the above-mentioned cell. The NAT-30 can be fused with a cell producing the antibody derived from humans, and has sensitivity to aminoputerine having 4X10<-7>M and further sensitivity to HAT culture medium. |
