OVALURATORY AGENT AND ITS PREPARATION

摘要

PURPOSE:Ethyl acetate is added to bran of adlay to extract its lipid fraction followed by gradient elution with n-hexane-ethyl acetate to give a phytosterol fatty acid ester active as an ovulratory free from side-effects in high yield. CONSTITUTION:A phytosterol fatty acid ester of the formula (R1 is residue of palmitic acid; R2 is methyl, ethyl) such as beta-cytosterol palmitate or campesterol plamitate is used as an active ingredient to give the objective ovulatory. The compound of the formula induces physiologically natural ovula tion without abnormality of sexual cycle. The compound of the formula is obtained by removing ethyl acetate form a mixture of lipid fraction adlay bran with ethyl acetate and another fraction aoluble in ethanol-ethyl acetate and subjecting the residue to silica-gel chromatography by gradient elution with n-hexane-ethyl acetate.