MULTI-STAGE EXTRACTION TREATMENT OF CELL OF MOLD BELONGING TO MORTIERELLA GENUS

摘要

PURPOSE:To extract and separate a lipid component in a highly refined state, in an improved extraction efficiency, by extracting the cells of mold belonging to Mortierella genus under specific condition, and carrying out the solid-liquid separation of the extract. CONSTITUTION:Cells of a mold belonging to Mortierella genus are disintegrated in an alcohol solvent in the presence of water by mechanical force. >=90% of the whole polar lipid contained in the cell and a part of neutral lipid are extracted and separated by this process. The extract is separated into the disintegrated cell component and the alcohol solvent component containing polar lipid by a solid-liquid separation step. The disintegrated cell component is again extracted with a hydrocarbon solvent to extract and separate the neutral lipid in high efficiency. The extraction product is separated into the microbial cells and the hydrocarbon solvent containing the neutral lipid by solid-liquid separation.