| 摘要 |
<p>A process for preparing a human cancer-specific monoclonal antibody, which comprises centrifuging a homogenized human cancer cell mixture, subjecting the supernatant to centrifugation in a discontinuous sucrose density gradient solution to collect a fraction gathering on the discontinuous plane, superposing the fraction on a continuous sugar density gradient solution, conducting ultracentrifugation to separate a fraction of 1.191 ς to 1.210 ς in density rich in a cancer-specific antigen, immunizing animals with this antigen, preparing a fused cell of the immunized spleen cell and myeloma cell, screening hybridoma capable of yielding the intended antibody, conducting a cloning procedure, and culturing the hybridoma. The resulting monoclonal antibody can provide the doctor with means for discovering cancer at its early stage, instructions on diagnosis and treatment of cancer, and means for monitoring recrudescence of cancer.</p> |
