| 摘要 |
Methods and vectors for the production of prokaryotic carbonyl-hydrolases in recombinant systems are created. The DNAs which code for such hydrolases are mutated in predetermined regions by known methods or by a new method of point mutagenesis with the aim of producing mutant hydrolases. Mutations of particular points in carbonyl- hydrolases, such as subtilisin, result in modifications of the oxidation stability, substrate specificity, Km, Kcat, Kcat/Km ratio, specific activity or activity-pH profiles. These mutated hydrolases are particularly useful in machine washing (laundry) compositions. The mutations in the genes which code for subtilisin or neutral protease of Bacillus provide in essence normally spore-forming Bacillus strains that are unable to excrete subtilisin or neutral protease. Said strains are useful in the recombinant synthesis of heterologous proteins. |
