| 摘要 |
PURPOSE:To cultivate the titled microorganism industrially efficiently, by detecting rise in pH caused by consumption of acetic acid resulting from fermentation in a medium containing an acetate mixed with a methane producing microorganism, neutralizing the medium with acetic acid, and keeping the pH at the fittest value. CONSTITUTION:A medium containing growth factors such as an N source, a phosphoric acid source, an S source, mineral, vitamin, etc. and an acetate is used, a culture container is filled with an anaerobic gas atmosphere such as N2, etc., the medium is mixed with a methane producing mold, a microorganism to form methane from acetic acid is fed to the medium, and cultivation is started at a temperature suitable for growth at the fittest pH. Then, as the cultivation is advanced, the microorganism is multiplied and acetic acid is consumed, the rising pH value is detected by a pH sensor, acetic acid is dropped to keep the fittest pH condition, and the cultivation is continued efficiently.
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