IMMUNOLOGIC ASSAY FOR DETERMINING PRESENCE OF A PHOSPHOLIPID IN BIOLOGICAL FLUID

摘要

An immunologic assay for determining the presence of one or more phospholipids in a biological fluid. The method includes adding an ethanolic solution of diacylphosphatidylcholine or alkyltrimethylammonium halide and cholesterol to the biological fluid forming a macromolecular aggregate complex suspension. To the macromolecular aggregate complex solution is then added either an additional quantity of the biological fluid or a buffer reagent. The product of the reaction is then added to antibody molecules to the phospholipid and examined to determine the presence of the phospholipids. The method is particularly useful in determining the presence of phosphatidylglycerol in a sample of amniotic fluid.