PROCESS FOR RECOVERING HUMAN IFN-BETA FROM A TRANSFORMED MICROORGANISM

摘要

A process for recovering IFN- beta from transformed bacteria comprising: disrupting the cell membranes of the bacteria; solubilizing the IFN- beta from the disruptate into an aqueous medium with a solubilizing agent such as sodium dodecyl sulfate; extracting the IFN- beta from the aqueous medium with 2-butanol, 2-methyl-butanol, or mixtures thereof under conditions that maintain phase separation between the aqueous meduim and the extractant; and isolating the IFN- beta from the extractant such as by precipitating the IFN- beta from an aqeous buffer mixture of the extractant by lowering the pH thereof.