发明名称 |
Process and materials for producing soluble biological mediators from white blood cells |
摘要 |
A process and cellular system for producing soluble biological mediators, including cytokines, lymphokines, monokines and interferons, and mRNA therefor, from white blood cells is provided. The process involves treating white blood cells with a mitogenic or antigenic agent in the presence of a controlled amount of red blood cells. Increases in production of the soluble biological mediator, gamma interferon, on the order of 5 to 10 fold have been achieved by maintaining the ratio of red blood cells to white blood cells between 10 to 1 and about 50 to 1. The process is particularly useful in producing HuIFN- gamma with PHA-P.
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申请公布号 |
US4591557(A) |
申请公布日期 |
1986.05.27 |
申请号 |
US19820446160 |
申请日期 |
1982.12.02 |
申请人 |
INTERFERON SCIENCES, INC. |
发明人 |
KEYES, LYNNE D.;TESTA, DOUGLAS |
分类号 |
C07K14/52;C07K14/57;(IPC1-7):C12P21/00;A01N1/02;C12P21/02;C12P19/34;C12N5/00;C12N5/02;C01G9/00 |
主分类号 |
C07K14/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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