| 摘要 |
NEW MATERIAL:Amylase gene designated as DNA base sequence 877-3326. USE:Secretion vector of Bacillus subtilis. PREPARATION:For example, Bacillus circulans F2 strain is cultured and treated with lysozyme to obtain a chromosome DNA, which is inserted into PLS 330-4. Separately, Bacillus subtilis MO7-2-31 strain is cultured to obtain TN-106 strain (Bacillus subtilis MO7-2-31 strain containing PLS 310DELTA6). The prepared plasmid is introduced into the above TN-106 strain by transformation. A composite plasmid containing amylase gene is separated from the resultant TN-501 strain to obtain purified composite plasmid (PTN 603) containing amylase gene. |
