| 摘要 |
The dispenser component has a well with an opening adapted to present a liq. reagent for withdrawal through the well. A means is provided to position a lens of liq., immiscible with the reagent, between the reagent and the opening of the well. - The dispenser has an inner surface and suitable dimensions which combine with the wetting characteristics of the reagent to form a concave reagent meniscus suitable to reliably position the lens of immiscible liq. on it. The lens so formed is centrally located by the concave shape of the meniscus. - The device allows long-term storage of unmixed ingredients and secure packaging for shipping. The dispenser is formed to prevent contamination risks and error in manual reagent prepn. The device is partic. suitable for repeated introduction of reagents into probes of automated analytical systems, e.g. for biological liqs. such as whole blood, serum or plasma. (0/10) EPAB- EP-109277 B A method of dispensing a liquid reagent and an immiscible liquid for an analysis system, wherein there is used an apparatus which comprises: (a) at least one reagent well (75, 85, 175, 275) having a first opening for withdrawal of liquid reagent (R1, R2) from said well therethrough, and a second opening of introduction of liquid reagent into said well; (b) at least one reagent reservoir (30,40,130) for containing a supply of liquid reagent, the or each reservoir co-operating with a respective reagent well for supply of reagent thereto and to maintain (in use) a standing level of liquid reagent in the well; (c) means for repeatedly releasing a controlled amount of reagent liquid from the or each reservoir through a passage (36,46,136) between the reservoir and its respective well to introduce the said controlled amount of reagent liquid into said respective well through its said second opening to replenish the well after withdrawal of liquid reagent therefrom; and wherein the (or each well is so dimensioned and shaped and has an inner surface such that when it contains aqueous liquid reagent, a quantity of immiscible liquid placed on the surface of the reagent liquid in the well forms a floating lens centrally within the well; and wherein said reagent and said immiscible liquid are dispensed from the or each well by aspiration through a probe placed in a said reagent well. (17pp) - EP-109277 B A method of dispensing a liquid reagent and an immiscible liquid for an analysis system, wherein there is used an apparatus which comprises: (a) at least one reagent well (75, 85, 175, 275) having a first opening for withdrawal of liquid reagent (R1, R2) from said well therethrough, and a second opening of introduction of liquid reagent into said well; (b) at least one reagent reservoir (30,40,130) for containing a supply of liquid reagent, the or each reservoir co-operating with a respective reagent well for supply of reagent thereto and to maintain (in use) a standing level of liquid reagent in the well; (c) means for repeatedly releasing a controlled amount of reagent liquid from the or each reservoir through a passage (36,46,136) between the reservoir and its respective well to introduce the said controlled amount of reagent liquid into said respective well through its said second opening to replenish the well after withdrawal of liquid reagent therefrom; and wherein the (or each well is so dimensioned and shaped and has an inner surface such that when it contains aqueous liquid reagent, a quantity of immiscible liquid placed on the surface of the reagent liquid in the well forms a floating lens centrally within the well; and wherein said reagent and said immiscible liquid are dispensed from the or each well by aspiration through a probe placed in a said reagent well. (17pp) GBAB- GB2130553 B A unitary integral reagent container comprising mears defining a reagent well having a first opening for withdrawal of liquid reagent from said well therethrough and a second opening for introduction of liquid reagent into said well; means for establishing a standing level of reagent in said reagent well; means for maintaining a lens of immiscible liquid on the surface of such reagent and within a defined area of such surface when such reagent is contained in said reagent well; means defining a reagent reservoir integral with said reagent well for containing a supply of reagent liquid; and means for repeatedly releasing a controlled amount of reagent liquid from said reagent reservoir through an opening between said reagent reservoir and reagent well and for introducing the controlled amount of reagent so-released into said reagent well through said second opening thereof. USAB- US4515753 A Integral reagent container comprises a series of wells each of which has an inlet and an outlet, a mechanism for keeping a lens of immiscible liqs. on the reagent surface, and a series of reservoirs connected to a respective well. The flow of reagent from the reservoirs are controlled. - The lens is pref. centrally positioned on the reagent surface, and has a hydrophilic inner surface. - (12pp) |
