摘要 |
Proteolytic enzymes, particularly papain, are used for rendering whole blood incoagulable. The treatment is preferably carried out at a pH of 7.2 with a ratio between enzyme and substrate, for instance, 1:200, and a volume ratio of 1:10. In the case of papain, a 1.6% solution is advantageously used. The incoagulable blood may be used to prepare a protein concentrate by precipitating the proteins with an agent, for instance, acetone or ethanol, preferably at 0 DEG C. in the presence of hydrochloric acid. |