摘要 |
<p>PURPOSE:To advance a reaction in a homogeneous phase and carry out separation after the reaction with ease, by carrying out an enzymic reaction in the presence of a substance capable of being a solvent in the supercritical state. CONSTITUTION:For example, lipase, e.g. Rhizopus delemar, is used as an enzyme, and a fat or oil, e.g. palm oil or beef tallow, is used as a substance subjected to the enzymic reaction. The enzymic reaction is carried out using carbon dioxide as a substance capable of being a solvent in the supercritical state. The lipase and the fat or oil are put into reaction vessels 1 and 1', and gaseous carbon dioxide (critical temperature; 31.1 deg.C, critical pressure; 73atm) is boosted with a liquid chromatographic pump 4, liquefied, fed to the reaction vessels 1 and 1' while controlling the pressure with valves 5-8, and reacted at about 32 deg.C under about 100atm pressure with stirring. After the reaction, the reaction vessels 1 and 1' are cooled with acetone-dry ice, and the reaction product is recovered in the dry ice. The dry ice is sublimed to extract and separate the aimed lipid from the residue.</p> |