发明名称 |
A PROCESS FOR DETECTING THE PRESENCE OF AN ANTIGEN IN A SPECIMEN |
摘要 |
<p>A process for detecting the presence of an antigen in a specimen is described, which process comprises: (A) contacting said specimen with a substrate coated with antibodies of said antigen, incubating the contacted substrate and washing the substrate; (B) contacting the washed material of step (A) with a hapten conjugated antibody against said antigen, incubating the so-contacted material and washing the so-incubated material; (C) contacting the washed material of step (B) with a radioactive material labeled or enzyme containing anti-hapten antibody, incubating the so-contacted material and washing the same; and (D) effecting radioimmunoassay if said antibody is radioactive or enzyme labeled immunoassay if said antibody contains an enzyme moiety. Quantitative determination of the antigen in the specimen is effected by comparing the counts of the radioimmunoassay or the concentration of enzyme against a standard as by photocolormetric methods.</p> |
申请公布号 |
EP0019277(B1) |
申请公布日期 |
1986.01.29 |
申请号 |
EP19800102687 |
申请日期 |
1980.05.16 |
申请人 |
NEW YORK BLOOD CENTER, INC. |
发明人 |
NEURATH, A. ROBERT;STRICK, NATHAN |
分类号 |
G01N33/532;G01N33/543;(IPC1-7):G01N33/543 |
主分类号 |
G01N33/532 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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