发明名称 |
Isolating cells expressing secreted proteins |
摘要 |
A method for identifying and isolating cells which produce secreted proteins. This method is based upon a specific characteristic or the expression level of the secreted protein by transiently capturing the secreted protein on the surface of an individual cell, allowing selection of rare cell clones from a heterogeneous population. Also provided is the use of this method to generate cells which produce a desired level of secreted protein or secreted protein of a particular characteristic(s), and organisms which possess such cells. In particular, the method allows rapid isolation of high expression recombinant antibody-producing cell lines, or may be applied directly to rapid isolation of specific hybridomas, or to the isolation of antibody-producing transgenic animals. This method is applicable for any cell which secretes protein.
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申请公布号 |
US2002168702(A1) |
申请公布日期 |
2002.11.14 |
申请号 |
US20020050279 |
申请日期 |
2002.01.16 |
申请人 |
FANDL JAMES P.;STAHL NEIL;CHEN GANG;YANCOPOULOS GEORGE D. |
发明人 |
FANDL JAMES P.;STAHL NEIL;CHEN GANG;YANCOPOULOS GEORGE D. |
分类号 |
A01K67/027;C07K14/315;C07K14/52;C07K16/28;C12N5/10;C12N15/09;C40B30/04;G01N33/569;G01N33/68;(IPC1-7):C12Q1/24 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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