| 摘要 |
PURPOSE:To obtain an antibody of high quality having higher affinity and specificity than those of a raw material antibody which is a polyclonal antibody, by isoelectric focusing of the raw material antibody. CONSTITUTION:A raw material antibody collected by giving an antigen to immunize an animal is subjected to isoelectric focusing to afford the aimed antibody having higher affinity and specificity than those of the raw material antibody which is not a simple antibody but an aggregate of many immunoglobulins having various specificities and affinities. So far as the raw material antibody is used as a mixture, averaged rather low specificity and affinity are produced. The isoelectric focusing is carried out by dispersing various amphoteric carriers having individual isoelectric points in a floating field, placing a spot of the raw material antibody at an optional point, applying electric charge thereto, moving the above-mentioned antibody to the positions of the respective intrinsic isoelectric points, and separating the antibody as a mixture or simple substance having the isoelectric points as a common factor. The simple substance exhibits higher affinity and specificity than those of the raw material antibody.
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