Specific polypeptide producing hybrid cell lines obtained from primary cells which are spontaneously capable of expressing said polypeptide or an equivalent polypeptide, process for obtaining the hybrid cells and their use in the production of said polypeptide.

摘要

Hybrid cells transformed or transformable by a cloned DNA sequence containing the sequence coding for a determined polypeptide, for example an immunogene polypeptide having vaccination properties to hepatitis B. They are characterized in that they contain on one hand at least a portion of the genotype of a primary cell lineage which are naturally favorable to the expression of said DNA sequence and on the other hand a genetic marker which enable them to grow in a selective medium or containing an active principle which is normally lethal for the cells from which the hybrid is issued, but unactivable by the polypeptide expressed by said genetic marker.