| 摘要 |
PURPOSE:To prepare inexpensively alanine from lactic acid, by conjugating lactic dehydrogenase with L-alanine dehydrogenase in an aqueous medium containing lactic acid, an ammonia donor, and nicotinamide adenine dinucleotide. CONSTITUTION:Escherichia coli (FERM P-7475) capable of producing lactic dehydrogenase, having DNA to which a gene to code vector DNA and L-alanine dehydrogenase is linked, capable of producing L-alanine dehydrogenase, is prepared. The mold is cultivated to prepare lactate dehydrogenase and L-alanine dehydrogenase, which are treated in an aqueous medium containing lactic acid, an ammonia donor, and nicotinamide adenine dinucletoide, to give alanine. |
